Plotting Excercise 4 - heatmap

These exercises cover the sections on Plotting in R PlottingInR.

Section 1

Please load the dateset SubsetRlog_7D.csv from "data/SubsetRlog_7D.csv"

• hint: use read.csv() function to load SubsetRlog_7D.csv. If you failed to import SubsetRlog_7D.csv to R, please check whether you set up your work directory properly by using getwd() and setwd()

1. use scale() function to perform the z-score transform on each genes

• hint: use ?scale to see how to use this function properly and you might need t() to perform this analysis properly

?scale
?t

2. use Heatmap() function from R bioconductor packages ComplexHeatmap and RColorBrewer to create the heatmap below:

note: TimePoint colour codes are from brewer.pal(10, "Paired")[c(9:10)] and Treatment colour codes are from brewer.pal(6, "Dark2")[c(1,2)]